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             Chain Reaction (qPCR)

         
         

Expression Profiling Services

Whether you are looking to determine the mechanism of action of your compound, elucidate the molecular pathways of a disease process or are merely looking to define the molecular phenotype of a particular cell or tissue type, put our Expression Profiling services to work for you.

Precision Biomarker Resources has the capacity to process samples in a 96-well format compatable with most high-throughput cellular screening projects allowing you to move seamlessly from compound library to high-content RNA expression data.

Precision Biomarker Resources' automated processing starts with purified nucleic acids and yields highly reproducable data. Our process includes seven quality control check points where our technicians measure more than fourteen parameters for each sample in an experiment. This attention to detail gives you the confidence and the power to compare newly generated data with your historical data sets.

Click here for Sample Submission Guidelines

A basic bioinformatics report is included with each experiment. This typically includes an annotated list of differentially expressed genes plus a quality control report containing starting sample quality measurements and a post-hybridization analysis of global chip parameters.

Click here for a sample Expression Bioinformatics Report (PDF)

3' Whole-Genome Expression Profiling

As the basis of more than 3,600 peer-reviewed publications, Affymetrix's 3' Expression Arrays set the gold standard for whole-genome Expression Profiling.

With our outstanding reproducibility and commitment to quality, Precision Biomarker Resources sets the standard for microarray processing services.

Our automated high-throughput processing minimizes technical variability to give you the confidence that the results you are seeing are biological effects and not technical artifacts. Your samples are too important to trust to just anyone. Call Precision Biomarker Resources today to arrange processing of your samples and let us turn your samples into the sort of quality data you can use.

Two-Cycle processing for experiments with Limited Sample Material

Sometimes your samples are just too rare or too difficult to get microgram quantities of RNA. Cells isolated from a flow cytometer or laser capture microdissected sample are time-consuming to isolate and often yield only the smallest amount of RNA. This need not put Expression Profiling out of reach for you. Precision Biomarker Resources technical staff has processed numerous samples where the starting material was measured in the tens to hundreds of nanograms. Call us today to discuss how we might be able to put the power of genomic microarrays to work for you and your limited material samples.

Whole Transcript or Exon Profiling

A recent advancement in RNA Expression Profiling is the ability to determine the expression of multiple splice variants in a sample in an unbiased fashion. Precision Biomarker Resources has the ability and the expertise to process your samples using Affymetrixs Exon arrays. Dig deeper into the molecular processes present in your samples. Call PBR today to discuss Expression Profiling on an Affymetrix Exon Array.

Expression Microarray Probe Strategy Diagram

Diagram courtesy of Affymetrix


Useful Guidelines for Sample Submission

Processing Type
Total RNA
260:280
Concentration
3' Standard Single Cycle
1-5 ug
>1.8
>0.50 ug/ul
Small Scale
10-100 ng
>1.8
-
Whole Transcript (Exon/Gene)
1-2 ug
>1.8
>1.0 ug/ul


A complete list of all expression arrays available from Affymetrix

Download our Expression Profiling Microarray Services brochure

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